Albeit simple and low cost, most of the LFAs are restricted to yes/no or subjective and interpretable semi-quantitative results. The flagship of POC devices is paper-based lateral flow assays (LFAs), where the paper 1 pumps the liquid through the system by capillarity, and the device provides the result after a few minutes and without user intervention. Introduction Point of care (POC) devices are conceived to transfer medical tests from central laboratories to the place of patient's care. immunoassays, with the aim of transferring them from central laboratories to the place of patient's care. Although demonstrated for glucose, SF microfluidic test strips may incorporate other optical bioassays, e.g. Additionally, SF films triple the durability of most stable cellulose-based sensors. This enhanced sensitivity results from the formation of SF–ABTS complexes, where SF becomes part of the bioassay. The bulk functionalization of SF with the enzymes glucose oxidase and peroxidase and the mediator ABTS produces colourless and transparent SF films that respond to blood glucose increasing 2.5 times the sensitivity of conventional ABTS-based assays. capillary pumping activity of 1.5 mm s −1, capacity to filter blood cells thanks to its small, but tuneable, porosity and enhanced biosensing sensitivity. Besides its excellent optical properties and unmet capacity to stabilize biomacromolecules, SF in test strips exhibits additional functions, i.e. In optical biosensing, silk fibroin (SF) appears as a promising alternative where other materials, such as paper, find limitations. E-mail: g Department of Electrical and Computer Engineering, Tufts University, Medford, MA 02155, USA h Department of Physics, Tufts University, Medford, MA 02155, USA i Laboratory for Living Devices, Tufts University, Medford, MA 02155, USA E-mail: Tel: +34935947700 b São Carlos Institute of Physics, University of São Paulo, São Carlos, SP, Brazil c Departament de Química, Universitat Autònoma de Barcelona Bellaterra (Barcelona), 08193, Spain d Instituto Murciano de Investigación y Desarrollo Agrario y Alimentario (IMIDA), La Alberca (Murcia), 30150, Spain e Institute of Chemistry, São Paulo State University (UNESP), Araraquara, SP 14801-970, Brazil f Silklab, Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA. * a a Instituto de Microelectrónica de Barcelona (IMB-CNM, CSIC), Bellaterra, Barcelona 08193, Spain. Lab Chip, 2021, 21, 608-615 Nanoporous silk films with capillary action and size-exclusion capacity for sensitive glucose determination in whole blood †
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